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                                                                                                                                                                                                                                              技術文章您現在的位置:首頁 > 技術文章 > 凈信冷凍研磨儀助力解決實驗對鼠尾組織樣品的前處理

                                                                                                                                                                                                                                              凈信冷凍研磨儀助力解決實驗對鼠尾組織樣品的前處理

                                                                                                                                                                                                                                              發布時間:2022-11-08   點擊次數:161次

                                                                                                                                                                                                                                                因客戶需求,此次的實驗樣品是小鼠的尾部組織,由于后續需要對其鼠尾組織提取蛋白及RNA的濃度,為了提高所提取樣品的蛋白及RNA的濃度,此次實驗需求:需要對樣品組織進行細磨,由于實驗操作中有涉及到蛋白、RNA提取,因此還需選用低溫研磨的操作方式。

                                                                                                                                                                                                                                                

                                                                                                                                                                                                                                                由于有提取液的存在,因此在此次實驗操作中,溫度不能太低,否則凍住液體會導致無法研磨的狀況出現,因此在經過反復對比后,用戶選用了上海凈信的冷凍研磨儀設備來處理鼠尾樣品組織。

                                                                                                                                                                                                                                                

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                                                                                                                                                                                                                                                客戶介紹:西湖大學,位于浙江省杭州市,是一所社會力量舉辦、國家重點支持的新型高等學校,前身為浙江西湖高等研究院。

                                                                                                                                                                                                                                                

                                                                                                                                                                                                                                                樣品組織前處理實驗操作: 

                                                                                                                                                                                                                                                1、準備實驗冷凍研磨設備一臺,金屬適配器一套以及離心管和鋼珠;  

                                                                                                                                                                                                                                                2、預冷模塊,把金屬適配器放到儀器內,打開壓縮機預冷;  

                                                                                                                                                                                                                                                3、取適量鼠尾組織、鋼珠、提取液一起放入離心管中,加入鋼珠后蓋子蓋上;  

                                                                                                                                                                                                                                                4、將完成預冷后的金屬模塊放入設備中固定放平后將離心管分別放入適配器對應孔位,蓋上適配器蓋,旋緊螺帽;  

                                                                                                                                                                                                                                                5、設置頻率以及時間后,啟動設備開始運轉;  

                                                                                                                                                                                                                                                6、磨好后打開儀器取出離心管,即可進行后續實驗。

                                                                                                                                                                                                                                                

                                                                                                                                                                                                                                                動物組織樣品研磨前后效果圖:

                                                                                                                                                                                                                                                

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                                                                                                                                                                                                                                                上圖為90s的研磨效果,其細度遠遠超出老師的預期。之前也在其他實驗室體驗過我們儀器,經過我們銷售人員協助搭配鋼珠也達到很好的效果。之前所有樣品都是人工處理,費時費力實驗周期非常長,所以選用凈信液氮冷凍研磨儀代替人工處理。效率高,效果好。非常符合老師的需求。

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